Evaluation of INNO-LiPA MYCOBACTERIA v2: Improved Reverse Hybridization Multiple DNA Probe Assay for Mycobacterial Identification
نویسندگان
چکیده
منابع مشابه
Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria
A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jensen and Middlebrook 7H9 media and identif...
متن کاملIdentification of mycobacteria by using INNO LiPA.
I have read with interest the recent paper of Suffys et al. (1) concerning the identification of 157 mycobacterium strains by using the INNO LiPA Mycobacteria (LiPA) assay. The same journal had published, 1 year ago, a similar paper, which I coauthored, on 238 strains (2), the results of which are now substantially confirmed. I feel, however, that Suffys et al. did not take it into due account;...
متن کاملRapid identification of Mycobacterium genavense with a new commercially available molecular test, INNO-LiPA MYCOBACTERIA v2.
We report a rare mesenteric localized Mycobacterium genavense infection in a severely immunocompromised human immunodeficiency virus-infected patient. An INNO-LiPA MYCOBACTERIA v2 test was performed directly on biopsy samples. This new molecular tool could be used for simultaneous identification of mycobacterium species from human specimens, but other studies are needed to validate our first re...
متن کاملEvaluation of INNO-LiPA assay for direct detection of mycobacteria in pulmonary and extrapulmonary specimens.
The INNO-LiPA Mycobacteria kit has been developed for detecting mycobacteria in liquid and solid cultures through amplification of the 16S-23S rRNA mycobacterial spacer region and the use of species-specific probes. The aim of this study was to verify the possible direct use of the kit on clinical samples. The study was performed retrospectively on a total of 129 specimens (104 pulmonary and 25...
متن کاملEvaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.
Two DNA strip assays, INNO-LiPA MYCOBACTERIA and GenoType Mykobakterien, were evaluated for identification of 81 Finnish mycobacterial isolates. The LiPA assay correctly identified 89.4% of the 66 isolates studied, and the GenoType assay identified 95.1% of 81 isolates. The GenoType assay had a wider selection of species and less stringent temperature requirements.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.9.4418-4420.2003